Volume 1, Issue 2 (6-2023)                   JPSAD 2023, 1(2): 42-46 | Back to browse issues page

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Nazem Shirazi M H, Soltani Majd N, Khodayari M. Molecular and biochemical characterization of Pseudomonas aeruginosa isolated from a Turkey flock. JPSAD 2023; 1 (2) :42-46
URL: http://jpsad.ir/article-1-41-en.html
1- Sana Avian Hospital, Tehran, Iran , nazemshraz@yahoo.com
2- Sana Avian Hospital, Tehran, Iran
3- Department of Avian Diseases, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
Abstract:   (174 Views)
Pseudomonas aeruginosa is a Gram-negative, aerobic, mostly motile, non-spore-forming, catalase-positive bacterium responsible for nosocomial infections, food and waterborne diseases in the human population, and infection of different organs in avian species. Because of the emergence of multidrug-resistant isolates in recent years, the occurrence of infection in pet avian species or poultry can lead to severe infection in the human population. This study reports an infection in turkey poults related to Pseudomonas aeruginosa. Overall, eight 4-day-old dead turkey chicks were presented to SANA Avian Hospital to diagnose the cause of death. For this purpose after necropsy, liver and bone marrow were sampled and cultured on blood agar. Then other complementary biochemical tests were performed to achieve a primary diagnosis. After the primary diagnosis of Pseudomonas aeruginosa infection, at the next step, PCR was used for definitive diagnosis and evaluation of virulence factors. Finally, the agar disk diffusion method determined antimicrobial resistance to some common medications, and results were interpreted by Clinical and Laboratory Standards Institute guidelines.
 
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Type of Study: others paper | Subject: Poultry Sciences
Received: 2023/09/21 | Accepted: 2023/10/14 | Published: 2023/12/6

References
1. Streeter K, Katouli M. Pseudomonas aeruginosa: a review of their pathogenesis and prevalence in clinical settings and the environment. Infection Epidemiology and Microbiology. 2016;2(1):25-32. [DOI:10.18869/modares.iem.2.1.25]
2. Urgancı NN, Yılmaz N, Alaşalvar GK, Yıldırım Z. Pseudomonas aeruginosa and Its Pathogenicity. Turkish Journal of Agriculture-Food Science and Technology. 2022;10(4):726-38. [DOI:10.24925/turjaf.v10i4.726-738.4986]
3. Kidd TJ, Gibson JS, Moss S, Greer RM, Cobbold RN, Wright JD, et al. Clonal complex Pseudomonas aeruginosa in horses. Veterinary microbiology. 2011;149(3-4):508-12. [DOI:10.1016/j.vetmic.2010.11.030] [PMID]
4. Poonsuk K, Chuanchuen R. Contribution of the MexXY multidrug efflux pump and other chromosomal mechanisms on aminoglycoside resistance in Pseudomonas aeruginosa isolates from canine and feline infections. Journal of Veterinary Medical Science. 2012;74(12):1575-82. [DOI:10.1292/jvms.12-0239] [PMID]
5. Abdul-Aziz T. Miscellaneous and sporadic bacterial infections. In: Swayne DE, editor. Diseases of Poultry. 14th ed: Wiley -Blackwell.; 2020. p. 1044-55.
6. Razmyar J, Zamani AH. An outbreak of yolk sac infection and dead-in-shell mortality in common canary (Serinus canaria) caused by Klebsiella pneumoniae. Iranian journal of veterinary research. 2016;17(2):141.
7. Meamar N, Razmyar J, Peighambari SM, Yazdani A. Drug Resistance Pattern of Pseudomonas aeruginosa Isolates Carrying MexAB-OprM Efflux Pump's Associated Genes in Companion Birds with Respiratory Infection. Iranian Journal of Veterinary Medicine. 2021;15(4).
8. Loughlin MF, Jones M, Lambert P. Pseudomonas aeruginosa cells adapted to benzalkonium chloride show resistance to other membrane-active agents but not to clinically relevant antibiotics. Journal of Antimicrobial Chemotherapy. 2002;49(4):631-9. [DOI:10.1093/jac/49.4.631] [PMID]
9. Abd El-Baky RM, Mandour SA, Ahmed EF, Hashem ZS, Sandle T, Mohamed DS. Virulence profiles of some Pseudomonas aeruginosa clinical isolates and their association with the suppression of Candida growth in polymicrobial infections. Plos one. 2020;15(12):e0243418. [DOI:10.1371/journal.pone.0243418] [PMID] []
10. Franzetti L, Scarpellini M. Characterisation of Pseudomonas spp. isolated from foods. Annals of microbiology. 2007;57:39-47. [DOI:10.1007/BF03175048]
11. Vaez H, Salehi-Abargouei A, Ghalehnoo ZR, Khademi F. Multidrug resistant Pseudomonas aeruginosa in Iran: A systematic review and metaanalysis. Journal of global infectious diseases. 2018;10(4):212. [DOI:10.4103/jgid.jgid_113_17] [PMID] []
12. Stafseth H, Mack W, Ryff J. Pseudomonas infection in turkeys. Poultry Science. 1940;19(2):126-30. [DOI:10.3382/ps.0190126]
13. Hafez H, Woernle H, Heil G. Pseudomonas aeruginosa infections in turkeys poults and treatment trials with apramycin. Berliner und München Tierärztliche Wochenschrift. 1987;100(2):48-51.
14. Marouf S, Li X, Salem HM, Ahmed ZS, Nader SM, Shaalan M, et al. Molecular detection of multidrug-resistant Pseudomonas aeruginosa of different avian sources with pathogenicity testing and in vitro evaluation of antibacterial efficacy of silver nanoparticles against multidrug-resistant P. aeruginosa. Poultry Science. 2023;102(10):102995. [DOI:10.1016/j.psj.2023.102995] [PMID] []

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